DOI:
VOLUME 1 – APRIL ISSUE 2
Ahmed Ali Mustafa*, Mubarak Siddig Hamad, Haifa A. A. Omer and Afaf R. Taher
Department of Botany and Microbiology, Faculty of Science, University of Gezira, Sudan
ABSTRACT
This study was carried out in Khartoum State, Sudan, during March, 2023. The plant of Corchorus tridens., locally known Malokheya, it was chosen for this plant because it is being used traditionally in treatment of many diseases. The objective of the present study was to evaluate the in vitro antibacterial and antioxidant activity of n-hexane, chloroform, ethyl acetate and methanolic extracts of aerial parts from Corchorus tridens.,. In addition, total phenol, flavonoids, tannins of these extracts were determined. Extracts from the plant was prepared by sequential maceration of dried aerial parts powder in solvents of increasing polarity. The antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria. Antioxidant activity were assessed based on the scavenging activity of the stable 2,2-Diphenyl- 1-picrylhydrazyl free radical (DPPH). Total polyphenolic, flavonoids and tannins contents were determined by spectrophotometric assays. Generally, the results of antimicrobial activity showed that extracts of the plant exhibited better antifungal activity than antibacterial. The results of antibacterial activity showed the highest in ethyl acetate and methanol extracts against Bacillus sabtilis and Escherichia coli (12±1.53 and 12±00mm respectively) flowed the methanol extract against Pseudomonas aeruginosa (11±2.52mm). While the highest antifungal activity showed the best value in methanolic extract against Candida albicans (19±1.00) and ethyl acetate against Aspergillus niger, (18±1.00mm). The highest scavenging radical activity was obtained from the methanol extract of aerial parts of Corchorus tridens., (64±0.02%) while the n-hexane extract gave lowest than the methanol extract (44±0.01). Quantitative analysis revealed that the total polyphenolic content was in the range of 88.79±0.04 to 50.21±0.04mg gallic acid equivalents/g with highest value shown in ethyl acetate extract. The total flavonoids content was in the range of 1034.19±0.03 to 128.23±0.00mg quercetin equivalents/g with highest value recorded from the ethyl acetate extract, while total tannins content was the range of 136.87±0.08 to 0.091±0.03mg tannic acid equivalents/g with highest value found in the methanol extract. In conclusion, the studied of plant was rich in bioactive agents with antioxidant, antifungal activities and total phenolic, flavonoids and tannins contents potential and could have interesting pharmaceutical and cosmetic applications.
Keywords:
Corchorus tridens.; Antimicrobial activity; Antioxidant activity; Total phenolic.
