EXPRESSION OF WT1 IN COLORECTAL ADENOCARCINOMA AND ITS ASSOCIATION WITH HISTOLOGICAL GRADE AND STAGE

Farjana Pervin Nupur*, Julekha Khatun, Silpi Pervin, Sonia Hossain, Sadia Refat Wahid, Jhuma Akter, Sadia Shirin, Shyla Sharmin Snigdha

ABSTRACT

Background: Colorectal carcinoma (CRC) is one of the most prevalent malignancies globally. Based on GLOBOCAN 2018 data, more than 2 million new cases of colorectal carcinoma (CRC) were recorded and caused around 1million deaths that year. Objectives: The aim of the present study was to determine the expression of WT1 in histomorphologically diagnosed colorectal adenocarcinoma and its association with histopathological grade and stage. Methodology: This cross-sectional observational study was conducted in the Department of Pathology, Dhaka Medical College (DMC), Dhaka, from March 2021 to February 2023. Large bowel resection specimens of 50 colorectal adenocarcinomas (CAC) were included in this study. Sections were stained with hematoxylin and eosin. Immuno staining with WT1 antibody was also done in Bangabandhu Sheikh Mujib Medical University (BSMMU). Relevant information was collected and recorded in a predesigned data sheet. The association of expressions of selected markers were evaluated with Chi-square test. P value significance was defined as p<0>0.05) between two groups. Out of six T4a tumor cases all 100% were WT1 positive. All N2 nodal status cases 9(100%) and 23 (95.8%) stage III cases were WT1 positive. Tumor extension, Lymph node status and stage were statistically significant (p<0>

Keywords:

WT1 immunomarker (WT1), immunohistochemistry (IHC), colorectal adenocarcinoma (CAC).

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