EXTRACTION OF CATHEPSIN S FROM BREAST CANCEROUS TISSUE (UNDERGO BREAST CONSERVING SURGERY ALONG WITH ADJUVANT RADIATION THERAPY.) AND ITS CHARACTERIZATION

Muhammad Zubair, Sadia Majeed, Ruman Aamer, *Rukhshan Khurshid, Noor Ulain, Amna Bakhtiar

ABSTRACT

Objective: Study was tried to extract/isolatecathepsin S from canceoroust issue received breast conserving surgery along with radiation therapy. Material and Methods: Human tissue (normal/cancerous) was obtained from surgery department of Sir Ganga Ram Hospital Lahore. Tissue was characterized as malignant. Extraction of cathepsin S was carried out. Identification of cathepsin S was carried out by electrophoresis.  Column chromatography was used to purify Cathepsin S. Level of cathepsin S was estimated using specific substrate and read photo metrically. Level of cathepsin was tried to associate with age (menarche / menopausal age) and clinic-pathological characteristics of patients. Results: Significantly high level of cathepsin S was observed in extract of breast cancerous tissue as compared to normal breast tissue. Level of cathepsin was high age at menarche <14years>Conclusion: Increase level of cathepsin S in breast cancer tissue, marking it a biomarker and potential therapeutic target. Higher level of cathepsinS in breast cancer patients with small tumor size and late stage of breast cancer may help to investigate cathepsin mediated tissue modification and alteration is important for research science and for clinical purposes, such as biomarkers.

Keywords:

Breast cancer tissue, cathepsin S; breast conserving surgery, radiation therapy.

Full Text Article Download Certificate